Neisseria gonorrhoeae by restriction fragment length polymorphisms
نویسندگان
چکیده
Objective-To characterise Neisseria gonorrhoeae isolates by restriction fragment length polymorphisms (RFLPs) in ribosomal RNA genes. Design-Generation of RFLP patterns by HincII restriction of rRNA genes followed by hybridisation with a nonradioactive labelled broad spectrum 16 + 23S rRNA gene probe. This typing method was developed and compared with MAb based serotyping. Specimens-Forty three randomly collected isolates from Bangkok (27 isolates) and Singapore (16 isolates) were studied. Results-The RFLP patterns generated were reproducible and highly discriminatory between strains. Analysis ofRFLPs produced by HincII restriction of rRNA genes established 9 patterns amongst the 43 isolates examined. Strains present within a common serovar could be further subdivided by RFLP typing. Identical RFLP patterns were found in some strains that belonged to various
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